Plasmid Transformation Of E. Coli - 999 Words

Plasmid transformation of E. coli using pVIB Savannah Jacobs April 4th, 2016 BIO 335 Spring 2016 Dr. Koester Abstract Since bacteria are haploid, asexually reproducing organisms it is important for these organisms to be able to accept genetic variability into their genome. A process called transformation, which involves absorbing small segments of DNA from deceased organisms in the natural world, does this. Transformation can also be mimicked in the laboratory using plasmid. Plasmids are small segments of DNA that occur in bacteria that allow us to regulate if transformation was successful. We attempted transformation of E. coli cells using plasmid called pVIB, which allows for luminescence and resistance to the antibiotic ampicillin, from Vibrio fischeri, however, we did not achieve a successful transformation. Introduction Some organisms, such as bacteria, have the ability to transform into a new form by picking up small segments of DNA from other organisms. This process is known as transformation and happens quite often in nature. Quite often organisms die and release their DNA into aqueous environments. The DNA is broken down but it is a while before it is fully destroyed allowing for bacteria cells and other organisms to transform their own DNA using the broken down segments from the deceased organism (Dodd). Transformation is an extremely important step in increasing genetic variation in organisms that reproduce asexually, allowing for them to make bothShow MoreRelatedGene Expression And Its Engineering1634 Words   |  7 PagesIntroduction Transformation is the process of allowing a cell to be able to accept a gene into itself and allow the gene to become part of the cell s whole, thus changing its form and expressing the gene s function. This process was first discovered by the British bacteriologist Frederick Griffith, who was able to transform Streptococcus pneumoniae into a different strain of Strep. in 1928 (1928). This discovery was pivotal in the way we understand gene expression and its engineering. An exampleRead MoreThe Discovery of Transformation by Frederick Grifith in Streptococcus Pneumonia704 Words   |  3 PagesThe discovery of transformation by Frederick Griffith in Streptococcus pneumonia has played an important role in how we are now able to introduce plasmid DNA molecules into cells. Transformation is the uptake of DNA molecules released from the donor cell by the recipient cell. It is one of the three ways bacteria are able to exchange genetic material. In Griffith’s experiment he introd uced mice to two different forms of S. pneumonia, one smooth, pathogenic and encapsulated and the other rough, nonpathogenicRead MoreTransformation Of Escherichia Bacteria And Dna1348 Words   |  6 PagesTransformation of Escherichia coli in different concentrations of Plasmid DNA Introduction This report discusses an experiment which students have to transform and plate competent Escherichia coli in different concentrations of plasmid DNA. This experiment uses four concentrations of plasmid DNA to perform four transformations. These concentrations are namely, 5  µl of distilled water, which acts as the control in this experiment; 2.5  µl of undiluted plasmid DNA; 1.0 µl of undiluted plasmid DNA; andRead MoreUse Of Antibiotic Resistance For A Large Number Of Infections1624 Words   |  7 PagesWard Period 6 January 22, 2015 Transformation Lab Abstract: Ampicillin is a beta-lactam antibiotic which can be used to treat a large number of infections. 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TransformationRead MoreBacteria Transformation in Biotechnology Essay1673 Words   |  7 PagesAbstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLOâ„ ¢ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media, it could be seen that theRead MoreThe Effect Of Puc18 And Lux Plasmids On Ampicillin Resistance Of Escherichia Coli1412 Words   |  6 PagesBSC 1010L U60 Effects of pUC18 and lux Plasmids on Ampicillin Resistance of Escherichia coli Abstract This experiment was designed to test and observe the transformation efficacy of the pUC18 and lux plasmids in making E. coli resistant to ampicillin. Both plasmids code for ampicillin resistance, however, the lux plasmid codes for a bioluminescence gene that is expressed if properly introduced into the bacteria’s genome. The E. coli cultures were mixed with a calcium chloride solutionRead MoreGenetic Expression In Gene Expression801 Words   |  4 Pagesinclude prokaryotes. Escherichia coli is a bacteria found in the human digestive system, preventing other pathogens from residing. Certain E. coli strains can be pathogenic and can lead to food poisoning, urinary tract infections, pneumonia, and gastrointestinal infections. Ampicillin is an antibiotic used to treat various bacterial infections including the ones mentioned. The antibiotic inhibits transpeptidase, an enzyme found in the cell walls of the bacterium. E. coli is preferably used consideringRead MoreTransformation Of Escherichia Coli With pGLO Plasmid1560 Words   |  7 Pagesï » ¿Transformation Of Escherichia Coli With pGLO Plasmid April 24, 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait, in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid, which carries a gene forRead MoreDifferent Methods, Bacteria, And Development Transduction, Transduction And Transformation Transduction934 Words   |  4 Pagestransduction and transformation Transduction is when a bacteriophage inserts it s DNA into the host bacteria cell, while conjugation is when the bacteria shares DNA through direct contact, eg. horizontal gene transfer. The third method, transformation, is the intake of external DNA into the cell (Chen and Dubnau, 2004). In order for bacteria to be able to take in extraneous DNA, they must first be in a state of competent (Hanahan, 1983). There are two types of transformation cells in transformation: natural

Power and Places Type Free Essays

We are going to talk about power and places type: - The power is necessarily an opposition between ruler and a ruled. There are many forms and places of power: Among the places of power, there are the White House, the Buckingham Palace, the Pentagon†¦ ? These places are powerful because they have political and military influence. Government is a governor who exercises his power through institutions such as the courts or prisons In this case, the governed are citizens who obey the laws of the government. We will write a custom essay sample on Power and Places Type or any similar topic only for you Order Now But sometimes there is abuse of power,and we see resistances to power, as when there was apartheid in South Africa. Indeed, there was a racial discrimination between blacks and whites. The great figure of the resistance in South Africa was Nelson Mandela: he fought all his life against discrimination against black, he was imprisoned for several years. ==========================================================================Among the forms of power, there is, for example, the power of the media. Indeed, the media play a very important role within today’s society. Media consists of books, television, movies, music, internet, radio, magazines, newspapers etc * Media have positive aspects: we can stay informed about political, social events very easily, you can have fun watching movies and listening to the music. (Through newspapers, TV, radio †¦) * But media have also bad/negative aspects. Indeed, today, it has become a great weapon to influence the opinions of individuals. For example, electronic media has the power to manipulate information, they can deny certain facts and expose another, they can broadcast in loop a topic to influence the vision of the mass of viewers (the presidential nominee can pay for more exposure on TV†¦) Media display the way of life for those who follows it. So media can influence the dressing manners of teenagers through theirs favorite film actors, etc. For example, children are specifically targeted in the advertisements. We teach them to eat Mc do’s burgers and drink coca cola. We can say that in this case, PEOPLE ARE BRAINWASHED BY MEDIA. In How to cite Power and Places Type, Papers

Negotiation Pre and Post Report Optimal Styles †Free Samples

Question: Discuss about the Negotiation Report Optimal Thinking Styles Answer: The kind of governments well demonstrates optimal thinking styles in various countries (Albaili, 1997). They reflect how one can govern their thinking levels. In theory, there are four different types of enlightened self-government: they include monarchic, hierarchic, oligarchic, and anarchic. The Monarchic kind of thinking projects an individual task by completely focusing on the project at hand till its completion. The person is mainly driven to a particular job till before moving to another hence being goal oriented. The Hierarchic individual has several projects and tasks hence the creation of hierarchical goals in the process (Saxena, 2013). One multiples things in a frame of time given by getting priorities set differently. The person tends to be more adaptive for the priorities laid down to be right and well done hence some things are more worthy of attention than others. The oligarchic individual has a preference for chores, projects, and situations that allow competition. Wi th multiple goals, one works under certain competitiveness so as to equally handle the important issues (Robert J and Ruzgis, 1994). Just like the hierarchically oriented person, one does multiple things within a given time setting but is confronted by the trouble of setting priorities for which to handle first. Competition enables one to adapt well to the tasks although one has problems when it comes to different priorities being set (Zhang, 2002). An anarchic individual is confronted with tasks, situations, and projects and one deal with them anyhow he or she finds to be pleasing. They may follow a systematic or ant routine procedure so far that results are reached. One often takes a casual approach to problems which are difficult for others to comprehend (Fisher and Ury, 2011). There are two levels of mental self-government which include the local and global. They exemplify a local individual who engages on tasks, specific to details and enjoys keeping track of the situations whi le a global person has situations that require his or her broad range of ideas and they never get worried about specific details. Adjustments From the theory, the most adopted style for negotiations is Hierarchic and an External scope of thinking. This encourages my thinking, therefore, adjusting my way by setting priorities in orders. I take the situations by interacting with the client to get some progress in the purchasing of the property. This makes me feel good at different stages of negotiating. It also enables me to have a lean to either side of the progress. There are two leanings of mental self-government: the liberal and conservative sides. The generous person has a fondness for tasks, projects, and situations that involve foreignness, thereby going beyond the current rules or measures, and total maximization of change. Sometimes, the individual may fancy change mainly for the sake of change, even when it is not needed. People displaying this style have an eye for new challenges and succeed on uncertainty. The conservative individual has an attachment to tasks, projects, and situations that involve devotion to pr esent rules and processes. One likes to lessen the adjustments hence avoid uncertainty. Some suggestions showed one's liking in the problem-solving process has an important bearing on consumer decision making. For example, the willingness for one to generate solutions adds some consideration in buying the product. BATNA and Reservation value client A reservation point occurs when there is the best alternative to the negotiated agreement (the BATNA). It is, therefore, more desirable to start or continue the negotiation process since the parties will not go beyond what was agreed. In this case, a total of $ 17,480,000.00 to be paid in 2 installments for the purchase of the property decided on April 28th, 2017. This is due on how the client has written or talked about the idea. The negotiation is centered on only one main idea and issues themes rather than the details of the property. This makes it hard to understand the whole process. BATNA and Reservation value other parties The BATNA and reservation value on other party introduces the bargaining range on other agreements and settling other properties. It can be positive or negative among the parties, and unless one changes his or her stand, therefore no agreement will be reached. In this situation, Farnsworth Property expresses an interest in 116 Davies Street, Liverpool, Sydney and another comparable alternative property in the area listed for sale by Uruz Holdings at a price of $21 million. This brings an alternative to negotiation, and the other party is eagerly awaiting the talk 116 Davies Street, Liverpool, Sydney either at a discounted price or for an even better price by 10%. Through developing a list of action, one conceivably takes a stand by improving the ideas and converting them to real options. ZOPA Exists in a potential agreement between the sides involved thus benefiting both rather than the alternative options available. Contract of the purchase is represented, and double checked so as to distribute the negotiation between the parties. There will be payment of the product by installations. The first installment will be $5,000,000.00 upon signature of Farnsworth accounting department for the agreed contract and the remaining cash valued at $12,480,000.00 completed within one month. Through the integration of the parties, it zeros into the bargaining range hence agreement are reached. Post Negotiation Report After a going through assertive and a cooperated negotiation with Pashendale Holdings Limited, I humbly wish to confirm that the purchase of 116 Davies Street, Liverpool, and Sydney is accepted. The seller Pashendale Holdings Ltd, have agreed through a signed document on the price agreed in Australian dollars, and hence dates of payment are set for the effectiveness of the deal. The value and conditions of the purchase of the property were agreed on April 28th, 2017 for a total of $ 17,480,000.00 to be paid in 2 installments. The first installment of $5,000,000.00 upon signature of the contract is scheduled on May 3rd, 2017 and the remaining value of $12,480,000.00 to be completed after one month from the first installment. After a broad search in the market, I can confirm that after several reservation values and bargaining ranges, the assessment criteria have made it easier for both the parties to reach an agreement therefore a successful purchase was reached, and the asking price was 10% below. The agent hoped that more purchase would be made under their new property trusts strategic plan so as to invest more in optimal and high performing assets all around the state. Reference Albaili, M. (1997). Differences Among Low?, Average? and High?achieving College Students on Learning and Study Strategies. Educational Psychology, 17(1-2), pp.171-177. Fisher, R. and Ury, W. (2011). Getting to Yes: Negotiating Agreement Without Giving In, 3rd ed. [eBook] New York: Penguin Books. Available at: https://www.beyondintractability.org/library/external-resource?biblio=23737. Robert J, S. and Ruzgis, P. (1994). "Thinking Styles: Theory and Assessment at the Interface Between Intelligence and Personality," 1st ed. New York: Cambridge University Press, pp.169-187. Saxena, S. (2013).Influence of Forms of Thinking Styles on Emotional Intelligence of Teacher Educators.IOSR Journal of Research Method in Education (IOSRJRME), 1(5), pp.30-33. Zhang, L. (2002). Thinking Styles and Cognitive Development.The Journal of Genetic Psychology, 163(2), pp.179-195.